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The effects of different levels of vitamin E and vitamin C in modified Beltsville extender on rooster post-thawed sperm quality


, : The effects of different levels of vitamin E and vitamin C in modified Beltsville extender on rooster post-thawed sperm quality. Cell and Tissue Banking 16(4): 587-592

Oxidative damage of sperm by means of reactive oxygen species generated by the cellular components of semen is one of the main reason of declined motility and fertility of sperm during the freeze-thawing process. This study was conducted to determine the influence of vitamin C and vitamin E on rooster post-thawed sperm motility, viability and malondialdehyde (MDA) level. Semen samples from 10 sexually-mature Ross 308 breeder roosters were collected and pooled, divided into nine equal parts and diluted with modified Beltsville extender containing with no antioxidants (control), or containing 100 (C100), 200 (C200), 400 (C400), 800 (C800) µg/mL vitamin C, and 2 (E2), 5 (E5), 10 (E10) and 15 (E15) µg/mL vitamin E. After thawing, total and progressive sperm motility, sperm viability and semen MDA level were assessed. The results shown that C200 and E5 extenders resulted in higher total motility (p < 0.05) compared to other extenders, with exception of E10 extender. Progressive motility was higher in E5 extender (p < 0.05) compared to other extenders, with exception of C200 and E10 extenders. Also, C200 and E5 extenders resulted in higher viability of post-thawed spermatozoa (p < 0.05) compared to other extenders. Finally, the results showed that MDA level was lower in C100 and C200 extenders compared to other extenders (p < 0.05), with exception of E5 extender. In conclusion, the results of the present study demonstrate that C200 and E5 can improve the function of post-thawed rooster spermatozoa.

US$19.90

PMID: 25779925

DOI: 10.1007/s10561-015-9506-9


Other references

Kitahara, H.; Kawata, M., 1987: Results of artificial and natural hybridization between Parnassius glacialis Butler and P. hoenei Schweitzer. Tyo To Ga, 384: 259-268

Zhou, M-Bo.; Wei, W-Ting.; Xie, Y-Xiang.; Lei, Y.; Li, J-Heng., 2010: Palladium-catalyzed cross-coupling of electron-poor terminal alkynes with arylboronic acids under ligand-free and aerobic conditions. Palladium-catalyzed cross-coupling reaction of terminal alkynes with arylboronic acids has been described. In the presence of Pd(OAc)(2) and Ag(2)O, a variety of terminal alkynes, including electron-poor terminal alkynes, smoothly underwent the re...

Sussman, Michael R., 1997: Arabidopsis pugilism Isolation of OKnock-outO plants for testing hypotheses on in situ function of plasma membrane proteins. FASEB Journal 11(9): A1126

Daszkiewicz,P.; Jedrzejewska,B.; Samojlik,T.; Banka,B., 2008: Game management and the last imperial hunts in Biaowieza Primeval Forest in the report of Mitrofan Golenko. The paper presents Polish translation of the article by Mitrofan Golenko (originally published in 1935 in Russian in "Vozrozhdenie" magazine) reporting the last hunts in Bialowieza Primeval Forest by the Russian tsar and his family that...

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Bacić, J., 1973: Endothorecic renal dystrophy. Lijecnicki Vjesnik 95(4): 202-204

Choudhuri, K.K.; Chakrabarty, A.N., 1969: Phosphatase activity of coagulase-positive and coagulase-negative staphylococci and their correlation with pathogenicity. Indian Journal of Experimental Biology 7(2): 123-124

Ashraf, M.I.; Khan, I.A., 2007: Expression of heterosis for some economic traits in upland cotton (Gossypium hirsutum, L.). Six F1 crosses of upland cotton and their reciprocals were evaluated during 2002-03 in Pakistan to assess the magnitude of heterosis and heterobeltiosis regarding number of bolls per plant, yield of seed cotton, ginning out turn and staple length....

Pedersen, W.L., 1984: Field evaluations of maize inbreds and population selections for resistance to Exserohilum turcicum, race 2. Eighty-five inbreds and 9 selections from the Iowa broad-based synthetic BS8 were evaluated for resistance to chlorotic-type lesions caused by a US isolate of E. turcicum [Setosphaeria turcica] race 2 which is virulent on germplasm with the Ht1 re...

Sudha, M.L.; Rajeswari, G.; Venkateswara Rao, G., 2014: Chemical composition, rheological, quality characteristics and storage stability of buns enriched with coriander and curry leaves. Effect of addition of normal (NL) and dehydrated (DL) curry leaves (Murraya koeniggi) and coriander leaves (Corinadrum sativum) in the ratio of 1:1 to refined wheat flour (WF) or a blend of refined wheat flour-whole wheat flour (WF-WWF, 1:1) on th...