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The glucagon like peptide 1 analogue, exendin-4, attenuates oxidative stress-induced retinal cell death in early diabetic rats through promoting Sirt1 and Sirt3 expression

, : The glucagon like peptide 1 analogue, exendin-4, attenuates oxidative stress-induced retinal cell death in early diabetic rats through promoting Sirt1 and Sirt3 expression. Experimental Eye Research 151: 203-211

This study was aimed to further investigate the possible mechanisms by which the glucagon like peptide 1 analogue, exendin-4 (EX4), protects rat retinal cells at the early stage of diabetes. EX4 was injected intravitreally into normal and early-stage streptozotocin-diabetic rats. Cell death, reactive oxygen species (ROS), and electroretinogram (ERG) were measured. Sirtuin (Sirt) mRNA and protein were analyzed. In retinas of diabetic rats 1 month after diabetes onset, cell death and ROS level increased significantly, and the b-wave amplitudes and OPs were significantly reduced. Four days after intravitreal EX4 treatment, retinal cell death and ROS level in retinas reduced significantly, and visual function was recovered. In the retinas of early-stage diabetic rats, the expressions of Sirt1 and Sirt3 were also found to be significantly decreased, and both were back to normal levels after intravitreal injection of EX4. In R28 cells, hydrogen peroxide (H2O2) treatment increased ROS and cell death and decreased Sirt1 and Sirt3. With the addition of EX4 into the culture system, the expressions of Sirt1 and Sirt3 were increased, and the H2O2-induced ROS and cell death were significantly reduced. These results confirm a mechanism for EX4 to protect retinal cells from diabetic damage and oxidative injury. EX4 reduces retinal cell death and ROS generation by upregulating Sirt1 and Sirt3 expressions in the retina of early-stage diabetic rats as well as in H2O2-treated R28 cells.


PMID: 27212443

DOI: 10.1016/j.exer.2016.05.002

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Longobardi, M.; Ranise, A.; Bondavalli, F.; Mariani, E.; Schenone, P.; Lampa, E.; Giasi, M.; Paone, G.; Mazzeo, V.; Marmo, E., 1981: Esters of 6 cis di alkylamino 1 3 3 trimethyl 2 oxa bi cyclo 2.2.2 octan 5 trans ols with hypotensive and other activities. Three series of esters of 6-cis-dialkylamino-1,3,3-trimethyl-2-oxabicyclo[2.2.2]octan-5-trans-ols (dialkylamino = pyrrolidino, piperidino, morpholino) are described. A number of these compounds showed strong hypotensive activity in rats, and infil...

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She, J.; Zhou, J.; Hu, Z.; Xia, Y., 2015: Clinical observation of atrial threshold monitoring algorithm: a single center experience. To observe the atrial capture management in an atrial threshold monitoring algorithm. By calculating the enabling rate of the atrial threshold monitoring algorithm and comparing atrial thresholds measured automatically and manually, we evaluate it...

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Sugiura, J.; Pasternack, E., 2009: Novel mechanical caliper image while drilling and borehole image analysis. Petrophysics (Houston, Tex.) 50.2

Eren Muhsin; Tasli Kemal, 2002: Kilop Cretaceous hardground (Kale, Gumushane, NE Turkey); description and origin. A hardground surface is well exposed in the Kilop area of Kale (Gumushane, NE Turkey) which forms part of the Eastern Pontides. Here, the hardground is underlain by shallow water Lower Cretaceous limestones and overlain by Upper Cretaceous red lim...

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Sakr, A.; Caracciolo, E.; Donohue, T., 2007: A 45-year-old woman with fibromuscular dysplasia and celiac disease. International Journal of Cardiology 118(2): E27-E30

Allen, T.M.; Hansen, C.; Martin, F.; Redemann, C.; Yau-Young, A., 1991: Liposomes containing synthetic lipid derivatives of poly(ethylene glycol) show prolonged circulation half-lives in vivo. Novel synthetic lipid derivatives of poly(ethylene glycol) (PEG) have been synthesized and tested for their ability to decrease uptake of liposomes into the mononuclear phagocyte system (MPS, reticuloendothelial system) in mice and to prolong circ...

Gilbertz, D.; Johnson, B., 1987: Response of bedding plants and weeds to herbicides. Journal of environmental horticulture 5(4): 158-162