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VNTR diversity in Yersinia pestis isolates from an animal challenge study reveals the potential for in vitro mutations during laboratory cultivation

, : VNTR diversity in Yersinia pestis isolates from an animal challenge study reveals the potential for in vitro mutations during laboratory cultivation. Infection, Genetics and Evolution 45(): 297-302

NlmCategory="UNASSIGNED">Underlying mutation rates and other evolutionary forces shape the population structure of bacteria in nature. Although easily overlooked, similar forces are at work in the laboratory and may influence observed mutations. Here, we investigated tissue samples and Yersinia pestis isolates from a rodent laboratory challenge with strain CO92 using whole genome sequencing and multi-locus variable-number tandem repeat (VNTR) analysis (MLVA). We identified six VNTR mutations that were found to have occurred in vitro during laboratory cultivation rather than in vivo during the rodent challenge. In contrast, no single nucleotide polymorphism (SNP) mutations were observed, either in vivo or in vitro. These results were consistent with previously published mutation rates and the calculated number of Y. pestis generations that occurred during the in vitro versus the in vivo portions of the experiment. When genotyping disease outbreaks, the potential for in vitro mutations should be considered, particularly when highly variable genetic markers such as VNTRs are used.


PMID: 27664903

DOI: 10.1016/j.meegid.2016.09.019

Other references

Neubauer, C., 1944: Myocarditis in acute infective diseases: A Review of 200 Cases. Archives of Disease in Childhood 19(100): 178-180

Smith, I.W.M.; Sage, A.M.; Donahue, N.M.; Herbst, E.; Quan, D., 2006: The temperature-dependence of rapid low temperature reactions: experiment, understanding and prediction. Despite the success of the CRESU (Cinétique de Réaction en Ecoulement Supersonique Uniforme) method in measuring rate coefficients for neutral-neutral reactions of radicals down close to the very low temperatures prevalent in dense interstellar...

Ramalho, F.S., 1977: Resistance of cultivars of soya bean, Glycine max (L.) Merril, to the pod borer Etiella zinckenella Treitschke, 1832. The resistance of 10 cultivars of soya bean to attack by Etiella zinckenella (Treitschke) was studied in the field at Petropolis, Pernambuco, Brazil, in 1976. There were significant differences between cultivars in the percentages of infested pods...

Wenzel, Edmund., 1994: Revision rheinischer Kafernachweise nach dem zweiten Supplementband zu den Kafern Mitteleuropas. Teil 4: Latridiidae, Mycetophagidae. (Ins., Col.). Mitteilungen der Arbeitsgemeinschaft Rheinischer Koleopterologen. 1 August; 43: 153-178

Baber, S.R.; Li, H.; Simakajornboon, N.; Kadowitz, P.J.; Ross-Ascuitto, N.T.; Ascuitto, R.J., 2005: Analysis of pulmonary vascular response to acute alveolar hypoxic challenge in young rabbits subjected to chronic hypoxia from birth. Chronic alveolar hypoxia induces vascular changes leading to pulmonary hypertension. We investigated the role of nitric oxide synthase (NOS) on basal pulmonary artery pressure (PAP) and on changes in PAP arising from an acute alveolar hypoxic chal...

Maksvytis H.; Simanovsky L.; Minnasian M.; Brody J.S.; Douglas W.H.J., 1982: In vitro studies of the lipid interstitial cell of the developing lung. Federation Proceedings 41(3): ABSTRACT 1054

Lean D.R.S.; Fricker H J.; Charlton M.N.; Cuhel R.L.; Pick F.R., 1987: The lake ontario usa canada life support system. Primary productivity provides most of the energy to support aquatic food chains. The rate is not only influenced by available solar radiation but also by temperature, availability of phosphorus, and the influence of physical mixing processes. The...

Kleiner, K.R.; Frett, J.J., 1996: A survey of heat tolerance in lima bean. Hortscience 31(6): 914

Monti, D.J.; Clevenger, J., 1999: Good news: men and women can talk with each other. Journal of the American Veterinary Medical Association 215(5): 0-1

Kehoe, E.J., 1989: Connectionist models of conditioning: A tutorial. Models containing networks of neuron-like units have become increasingly prominent in the study of both cognitive psychology and artificial intelligence. This article describes the basic features of connectionist models and provides an illustrativ...