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Vaccinia Virus Infection Requires Maturation of Macropinosomes

, : Vaccinia Virus Infection Requires Maturation of Macropinosomes. Traffic 16(8): 814-831

The prototypic poxvirus, vaccinia virus (VACV), occurs in two infectious forms, mature virions (MVs) and extracellular virions (EVs). Both enter HeLa cells by inducing macropinocytic uptake. Using confocal microscopy, live-cell imaging, targeted RNAi screening and perturbants of endosome maturation, we analyzed the properties and maturation pathway of the macropinocytic vacuoles containing VACV MVs in HeLa cells. The vacuoles first acquired markers of early endosomes [Rab5, early endosome antigen 1 and phosphatidylinositol(3)P]. Prior to release of virus cores into the cytoplasm, they contained markers of late endosomes and lysosomes (Rab7a, lysosome-associated membrane protein 1 and sorting nexin 3). RNAi screening of endocytic cell factors emphasized the importance of late compartments for VACV infection. Follow-up perturbation analysis showed that infection required Rab7a and PIKfyve, confirming that VACV is a late-penetrating virus dependent on macropinosome maturation. VACV EV infection was inhibited by depletion of many of the same factors, indicating that both infectious particle forms share the need for late vacuolar conditions for penetration.


PMID: 25869659

DOI: 10.1111/tra.12290

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Watzinger, N.; Lund, G.K.; Saeed, M.; Reddy, G.P.; Araoz, P.A.; Yang, M.; Schwartz, A.B.; Bedigian, M.; Higgins, C.B., 2005: Myocardial blood flow in patients with dilated cardiomyopathy: quantitative assessment with velocity-encoded cine magnetic resonance imaging of the coronary sinus. To quantify global myocardial perfusion using magnetic resonance imaging (MRI) in patients with heart failure due to idiopathic dilated cardiomyopathy (IDC) and to compare myocardial perfusion and microvascular reactivity with healthy subjects. A...

Miovski, D.; Popovski, T., 1970: Cataract extraction by means of freezing. Godisen Zbornik Na Medicinskiot Fakultet Vo Skopje 16: 165-169

Davidson, W.M., 1924: Observations and Experiments on the Dispersion of the Convergent Lady-beetle (Hippodamia convergens, Gnerin) in California. In order to test the value of the importations of Hippodamia convergens, Guer., from the Sierra Nevada into Imperial Valley, a number of marked beetles were liberated. Only a small fraction of the marked individuals were subsequently recovered, an...

Rothschild, M. de.; Neuville, H., 1907: Sur une dent d'origiue enigmatique. Archives de Zoologie Paris (Ser 4: 7 271-333

Simon, C., 2013: Q&A: Celeste Simon on hypoxia-cancer links. Interviewed by Suzanne Rose. M. Celeste Simon, PhD, discusses connections between abnormal hypoxia levels and cancer, as well as her research on hypoxia-inducible factor transcription factors, which mediate responses to hypoxic stress.

Rudner, M.; Rönnberg, J., 2008: Explicit processing demands reveal language modality-specific organization of working memory. The working memory model for Ease of Language Understanding (ELU) predicts that processing differences between language modalities emerge when cognitive demands are explicit. This prediction was tested in three working memory experiments with part...

Sakurakichi, K.; Tsuchiya, H.; Uehara, K.; Yamashiro, T.; Tomita, K.; Azuma, Y., 2004: Effects of timing of low-intensity pulsed ultrasound on distraction osteogenesis. We investigated the effects of low-intensity pulsed ultrasound (LIPUS; 30 mW/cm2 spatial and temporal average) on the timing of LIPUS treatment in distraction osteogenesis. Lengthening of the right tibia was performed in 75 male Japanese white rab...

Murano, G., 1976: Cryptorchism. Views of the president of the round table. Minerva Pediatrica 28(23): 1496-1498

Buriak, V.P., 1980: Spectral characteristics of drug agents that contain oxygen heteroatoms in the molecule. I. The UV spectra of 5-nitrofuran derivative drug agents. Farmatsevtychnyi Zhurnal: 52-55

Naumenko, E.V.; Belyaev, D.K., 1980: Neuroendocrine mechanisms in animal domestication. A review of Soviet work on the silver fox, with brief reference to fine-wooled sheep.