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Vector promoters used in Klebsiella pneumoniae


, : Vector promoters used in Klebsiella pneumoniae. Biotechnology and Applied Biochemistry 63(5): 734-739

NlmCategory="UNASSIGNED">Much effort has been devoted to the metabolic engineering of Klebsiella pneumoniae; however, our knowledge of the actual expression level of promoters used in K. pneumoniae is limited. In this study, the expression levels of three promoters were compared systematically by using the lacZ reporter gene with different carbon sources in K. pneumoniae. The results showed that, although promoters PT5 and Ptac designed for Escherichia coli were functional, PT5 appeared more efficient and the induction/repression ratio of Ptac was decreased extremely in K. pneumoniae. The basal level of Ptac for lacZ expression reached 396.5 U/mg, which was 9.5-fold higher compared with PT5 in LB medium, indicating Ptac can be used as an efficient "constitutive" promoter as well as an efficient induced promoter in K. pneumoniae. In different carbon sources medium, a newly constructed endogenous constitutive Pbud proved to be a stable and weak promoter. On the basis of our data, a set of Pbud and Ptac promoters could meet the broad range (about 1,000 orders of magnitude) of gene expression needed for engineered K. pneumoniae in glycerol-based medium.

US$19.90

PMID: 26234465

DOI: 10.1002/bab.1423


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Buchgraber, M.U.berth, F.A.klam, E., 2000: Comparison of HPLC and GLC techniques for the determination of the triglyceride profile of cocoa butter. Current methods for the authentication of cocoa butter (CB) are mainly based on a knowledge of its triglyceride (TG) composition. The performances of capillary GLC and nonaqueous HPLC with an evaporative light-scattering detector (ELSD) for the qu...

Hoerner, L., 1989: Meeting on continuing education of the Basel Intensive Care Group. How can work satisfaction be maintained?. Krankenpflege. Soins Infirmiers 82(12): 69-70

Mcmahon R.; Neal C., 1990: Aluminum dis equilibrium solubility controls in scottish uk acidic catchments. Soil and stream water aluminium and hydrogen ion data are presented for three Scottish catchments that are both acidic and acid sensitive. Stream waters have pH values ranging from 3.9 to 7.1 and aluminium concentrations in the range 0.07 to 129 ....

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Kummer, V., 2002: Rutstroemia fruticeti und Velutarina rufoolivacea: Zwei wenig beachtete Besiedler abgestorbener Rubus fruticosus-Ruten. The occurrence of the ascomycetes Rutstroemia fruticeti and Velutarina rufoolivacea in Germany and especially Brandenburg are presented. Both species were collected by the author several times mainly in Brandenburg during the winter half-years of...

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