geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Viable but non-culturable state and toxin gene expression of enterohemorrhagic Escherichia coli O157 under cryopreservation


, : Viable but non-culturable state and toxin gene expression of enterohemorrhagic Escherichia coli O157 under cryopreservation. Research in Microbiology (): -

NlmCategory="UNASSIGNED">As major food-borne pathogens worldwide, Escherichia coli are capable of toxin production directly causing severe human disease. However, routine methods are incapable of detecting viable but non-culturable (VBNC) bacteria in food products and raw materials, leading to false-negative identification. In this study, VBNC E. coli O157 strains were acquired after cryopreservation at -20 °C, with and without freeze-thawing; morphology was observed to be of shorter rod-shape, and toxin expression remained at relatively high levels. PMA-PCR assay for VBNC detection was also validated. Therefore, these results suggest that VBNC E. coli O157 strains may represent a strong threat to public health and food safety.

US$19.90

PMID: 27884785

DOI: 10.1016/j.resmic.2016.11.002


Other references

Furuta, H.; Morimoto, T.; Osuka, A., 2004: Structures and ligand exchange of N-confused porphyrin dimer complexes with group 12 metals. N-confused 5,20-diphenylporphyrin (NCDPP, 1) formed 2:2 dimer complexes with group 12 metals both in the solid state and in solution. X-ray single-crystal analyses of the Zn(II) and Cd(II) complexes (7, 8) revealed that each metal ion is coordinat...

Rafii M.; Firooznia H.; Golimbu C.; Mccauley D.I., 1984: Hematogenous osteomyelitis with fat fluid level shown by computed tomography. Radiology 153(2): 493-494

Kang Zaibin., 1984: Descriptions of three new species of the genus Tricula from Hubei Province, China. Oceanologia et Limnologia Sinica, 154: 299-309

Rhamhalinghan, M., 1989: Variations in the internal temperatures of melanics and typicals of coccinella septempunctata l. coleoptera coccinellidae. In the absence of radiant heat source, the body temperature of the lady beetles closely corresponds to air temperature. However, above 24.degree. C the internal temperature decreases, presumably due to evaporation. In the presence of radiant heat...

Tufail M.; Siddiqui M.S.; Ali S.; Mirza N.M.; Ahmad N.; Zafar M.S.; Zafar F.I., 1991: Investigation of gamma ray activity and radiological hazards of the bricks fabricated around lahore pakistan. Employing state of art measurement techniques and software, gamma-ray activity due to 224Ra, 232Th and 40K have been measured from bricks fabricated around Lahore, Pakistan. External and internal hazards due to dose rate from radioactivity of bric...

Rouaix, S., 1960: De quelques faunes des Gres inferieurs sur la bordure septentrionale du Hoggar (Sahara). Bullgeol. Fr: 796-802

Tan, A.; Lan, Z-xiu.; Xie, B-jun.; Wang, Y-cun., 2005: Effect of combined decompression operation on middle and late stage cerebral herniation. To observe the effect of combined decompression operation on patients with severe traumatic brain injury complicated by tentorial cerebral herniation. Ninety-seven patients with an admission Glasgow Coma Scale score 3-5 were randomly divided into...

Cheng, Y.; Zhou, J.; Wang, M.; Liu, Y.; Guo, B.; Chen, B., 2016: Single-shot multi-reaction monitoring of intact marker conjugates for quantitative profiling of human major microsomal glucuronidations and its utility to screen inhibitors from medicinal herbs. NlmCategory="UNASSIGNED">UDP-glucuronosyltransferase (UGT) is a polymorphic family of conjugating enzymes responsible for the elimination of a myriad of xenobiotics and endogenous compounds. The precise reaction phenotyping of this mu...

East, H., 1975: Agris. I have discussed Chan Chan in terms of its political and economic characteristics because state organization had a pervasive impact on the growth and structure of the settlement. In this sense the capital of Chimor resembles Cuzco the Inca capital...

Scharschmidt, L.A.; Gibbons, N.B.; McGarry, L.; Berger, P.; Axelrod, M.; Janis, R.; Ko, Y.H., 1987: Effects of dietary fish oil on renal insufficiency in rats with subtotal nephrectomy. We studied the effects of fish oil on the progression of renal insufficiency in rats with subtotal nephrectomy. Five weeks after a 1-2/3 nephrectomy, sixteen rats were fed two different diets which differed only in fat composition. Lipid in the co...