geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Visualization of Nanofibrillar Cellulose in Biological Tissues Using a Biotinylated Carbohydrate Binding Module of β-1,4-Glycanase


, : Visualization of Nanofibrillar Cellulose in Biological Tissues Using a Biotinylated Carbohydrate Binding Module of β-1,4-Glycanase. Chemical Research in Toxicology 28(8): 1627-1635

Nanofibrillar cellulose is a very promising innovation with diverse potential applications including high quality paper, coatings, and drug delivery carriers. The production of nanofibrillar cellulose on an industrial scale may lead to increased exposure to nanofibrillar cellulose both in the working environment and the general environment. Assessment of the potential health effects following exposure to nanofibrillar cellulose is therefore required. However, as nanofibrillar cellulose primarily consists of glucose moieties, detection of nanofibrillar cellulose in biological tissues is difficult. We have developed a simple and robust method for specific and sensitive detection of cellulose fibers, including nanofibrillar cellulose, in biological tissue, using a biotinylated carbohydrate binding module (CBM) of β-1,4-glycanase (EXG:CBM) from the bacterium Cellulomonas fimi. EXG:CBM was expressed in Eschericia coli, purified, and biotinylated. EXG:CBM was shown to bind quantitatively to five different cellulose fibers including four different nanofibrillar celluloses. Biotinylated EXG:CBM was used to visualize cellulose fibers by either fluorescence- or horse radish peroxidase (HRP)-tagged avidin labeling. The HRP-EXG:CBM complex was used to visualize cellulose fibers in both cryopreserved and paraffin embedded lung tissue from mice dosed by pharyngeal aspiration with 10-200 μg/mouse. Detection was shown to be highly specific, and the assay appeared very robust. The present method represents a novel concept for the design of simple, robust, and highly specific detection methods for the detection of nanomaterials, which are otherwise difficult to visualize.

US$19.90

PMID: 26208679

DOI: 10.1021/acs.chemrestox.5b00271


Other references

Liu, S.; Du, Z.; Li, P.; Li, F., 2012: Sensitive colorimetric visualization of dihydronicotinamide adenine dinucleotide based on anti-aggregation of gold nanoparticles via boronic acid-diol binding. A facile, highly sensitive colorimetric strategy for dihydronicotinamide adenine dinucleotide (NADH) detection is proposed based on anti-aggregation of gold nanoparticles (AuNPs) via boronic acid-diol binding chemistry. The aggregation agent, 4-me...

E.N.wihi, H.M.; Mihas, A.A., 1995: The role of nitric oxide in the pathogenesis of portal hypertensive gastropathy. Gastroenterology 108(4 SUPPL ): A1062

Sorenson J.C., 1991: On the relationship of phenological strategy to ecological success the case of broomsedge in hawaii. Andropogon virginicus L. (broomsedge) is a perennial bunchgrass of temperate origin which was introduced to the Hawaiian Islands early in the 20th Century. Since its introduction broomsedge has become the dominant species of savanna vegetation on...

Ozgur, Y.; Ikiz, S.; Carioglu, B.; Ilgaz, A.; Takai, S., 2000: Two cases of dead foals associated with Rhodococcus equi pneumonia in Turkey. Rhodococcus equi has been recognized as an equine pathogen since 1923 and is a worldwide pathogen in foals. Up to now, there has been one report on R. equi infection in an Arabian foal in Turkey. We report here two cases of R. equi pneumonia diagn...

Kamei Takeshi, 1989: Dilatancy characteristics of normally consolidated cohesive soils. Coefficient of dilatancy of clays is shown to be linearly related to their plasticity index. Undrained test results on twelve soils comprising natural reconstituted clays and clay-sand mixtures form the basis of the proposed correlation.--Modified...

Anonymous, 2011: The effects of aging on low and intermediate stages of form processing. This study explored the effects of healthy aging on the early stages of the processing of contours and shapes. Forty-two adults participated. Participants were divided into three groups: 16 young adults (19 to 34 years, mean = 27); 12 middle aged...

Tsuda K., 1981: Ultrastructural observations of the liver in alpha 1 anti trypsin deficiency pi mnichinan. Japanese Journal Of Clinical Electron Microscopy6: 742-743

Asmussen M.; Wright P B.; Abeler V., 1980: Primary cancer of the fallopian tube in norway 1952 1975. Acta Obstetricia et Gynecologica Scandinavica Supplement (93): 96

Eyding, D.; Schweigart, G.; Eysel, U.T., 2002: Spatio-temporal plasticity of cortical receptive fields in response to repetitive visual stimulation in the adult cat. Many psychophysical experiments on perceptual learning in humans show increases of performance that are most probably based on functions of early visual cortical areas. Long-term plasticity of the primary visual cortex has so far been shown in viv...

de Silva, N.Lakshitha.; Gooneratne, L.; Wijewickrama, E., 2017: Acute myocardial infarction associated with thrombotic microangiopathy following a hump-nosed viper bite: a case report. Hump-nosed viper bite is the commonest cause of venomous snakebite in Sri Lanka. Despite initially being considered a moderately venomous snake more recent reports have revealed that it could cause significant systemic envenoming leading to coagul...