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Xenon Combined with Therapeutic Hypothermia Is Not Neuroprotective after Severe Hypoxia-Ischemia in Neonatal Rats


, : Xenon Combined with Therapeutic Hypothermia Is Not Neuroprotective after Severe Hypoxia-Ischemia in Neonatal Rats. Plos One 11(6): E0156759-E0156759

Therapeutic hypothermia (TH) is standard treatment following perinatal asphyxia in newborn infants. Experimentally, TH is neuroprotective after moderate hypoxia-ischemia (HI) in seven-day-old (P7) rats. However, TH is not neuroprotective after severe HI. After a moderate HI insult in newborn brain injury models, the anesthetic gas xenon (Xe) doubles TH neuroprotection. The aim of this study was to examine whether combining Xe and TH is neuroprotective as applied in a P7 rat model of severe HI. 120 P7 rat pups underwent a severe HI insult; unilateral carotid artery ligation followed by hypoxia (8% O2 for 150min at experimental normothermia (NT-37: Trectal 37°C). Surviving pups were randomised to immediate NT-37 for 5h (n = 36), immediate TH-32: Trectal 32°C for 5h (n = 25) or immediate TH-32 plus 50% inhaled Xe for 5h (n = 24). Pups were sacrificed after one week of survival. Relative area loss of the ligated hemisphere was measured, and neurons in the subventricular zone of this injured hemisphere were counted, to quantify brain damage. Following the HI insult, median (interquartile range, IQR) hemispheric brain area loss was similar in all groups: 63.5% (55.5-75.0) for NT-37 group, 65.0% (57.0-65.0) for TH-32 group, and 66.5% (59.0-72.0) for TH-32+Xe50% group (not significant). Correspondingly, there was no difference in neuronal cell count (NeuN marker) in the subventricular zone across the three treatment groups. Immediate therapeutic hypothermia with or without additional 50% inhaled Xe, does not provide neuroprotection one week after severe HI brain injury in the P7 neonatal rat. This model aims to mimic the clinical situation in severely asphyxiated neonates and treatment these newborns remains an ongoing challenge.

US$19.90

PMID: 27253085

DOI: 10.1371/journal.pone.0156759


Other references

Gross, A., 1954: Role of a local point of irritation in the production in the white rat of an experimental asthmatic attack by intraperitoneal injection of ovalbumin. Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales 148(1-2): 95-97

Ebel, H., 1981: The psychohistory of history: a symposium. Journal of Psychohistory 8(3): 259-279

Kusano T.; Fukuyama K., 1989: Breeding activity of a stream breeding frog rana sp. Matsui, M , T Hikida And R C Goris (Ed ) Current Herpetology in East Asia; Second Japan-China Herpetological Symposium, Kyoto, Japan, July 1988 Ix+521p Herpetological Society Of Japan: Kyoto, Japan Illus Maps : 314-322

Arik; Gabay, 1994: Unification of gauge couplings in a Kaluza-Klein theory with two internal manifolds. Physical Review. D, Particles and Fields 49(10): 5604-5606

Sarkies, J.W., 1992: Trachoma still undefeated. British Journal of Ophthalmology 76(1): 61-61

Nikolic, M.; Cesco, S.; Romheld, V.; Varanini, Z.; Pinton, R., 2003: Uptake of iron (59Fe) complexed to water-extractable humic substances by sunflower leaves. This experiment evaluated the ability of leaves to utilize Fe supplied as a complex with water-extractable humic substances (WEHS) and the long-distance transport of 59Fe applied to sections of fully expanded leaves of intact sunflower (Helianthus...

Grodzinskaia, K.; Mandrovskaia, N.; Malakha, I.; Tyrygina, G.; Babenko, I., 1981: Change in the amount of available for plants phosphorus and potassium in volcanic rock caused by its inoculation with Achromobacter delicatulus. Mikrobiologicheskii zhurnal 43(4): 433-436

Kawalilak, C.E.; Johnston, J.D.; Olszynski, W.P.; Leswick, D.A.; Kontulainen, S.A., 2015: Comparison of short-term in vivo precision of bone density and microarchitecture at the distal radius and tibia between postmenopausal women and young adults. The purpose was to assess whether precision of bone properties derived via the use of high-resolution peripheral quantitative computed tomography (HR-pQCT) differs between postmenopausal women and young adults. Using HR-pQCT, we scanned the distal...

Keyna, U.; Beck Engeser, G.B.; Jack, H.M., 1995: Control of lambda-5 during B cell development. 9TH INTERNATIONAL CONGRESS OF IMMUNOLOGY [Author] The 9th International Congress of Immunology : 274

Baele G.; D.V.s M.; Huble F.; Elewaut A.; Barbier F., 1984: Influence of injection sclero therapy of esophageal varices in liver cirrhosis on the hemostatic system. Haemostasis 14(1): 131