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mRNA bound to the 30S subunit is a HigB toxin substrate

, : mRNA bound to the 30S subunit is a HigB toxin substrate. Rna 22(8): 1261-1270

Activation of bacterial toxins during stress results in cleavage of mRNAs in the context of the ribosome. These toxins are thought to function as global translational inhibitors yet recent studies suggest each may have distinct mRNA specificities that result in selective translation for bacterial survival. Here we demonstrate that mRNA in the context of a bacterial 30S subunit is sufficient for ribosome-dependent toxin HigB endonucleolytic activity, suggesting that HigB interferes with the initiation step of translation. We determined the X-ray crystal structure of HigB bound to the 30S, revealing that two solvent-exposed clusters of HigB basic residues directly interact with 30S 16S rRNA helices 18, 30, and 31. We further show that these HigB residues are essential for ribosome recognition and function. Comparison with other ribosome-dependent toxins RelE and YoeB reveals that each interacts with similar features of the 30S aminoacyl (A) site yet does so through presentation of diverse structural motifs.


PMID: 27307497

DOI: 10.1261/rna.056218.116

Other references

Jousset D.Bellesme,., 1879: Recherches sur le foie des Mollusques cephalopodes. JOUSSET DE BELLESME states that in the Cephalopods the secretion of the liver only transforms the albuminoid matter, having no effect on the grease and amylon, and therefore rather resembles the pancreatic secretion of the Vertebrate. C. R. lxxxvi...

Lyubartseva, L.A.; Angarskaya, M.A.; Sokolova, V.E., 1965: Effect of some new glycosides and aglycones from Convallaria on the central nervous system. The paper offers data on the influence exercised upon the central nervous system by the lily-of-the-valley glycosides and aglycones: convallatoxin and desgluco-cheirotoxin (aglycone strophanthidin), convaleatoxol (aglycone strophanthidin), and loc...

Maupin, C., 1977: Donnees micropaleontologiques nouvelles et precisions stratigraphiques sur le Lias du Kef Ben Chikr Bou Rouhou et du Kef Toumiette nord (chaine calcaire kabyle-nord du ConstantinoisAlgerie). Revue Micropaleont, 202: 91-99

Fazzi, A.; Soares, N.; Pessoa, C.R., 1969: Surgical treatment of recurring dislocation of the patella. Amb 15(7): 309-314

Kozynda Y.O.; Kritsuk I.N.; Sen kin V.Y., 1986: X-ray data analysis during geochemical prospecting for sulfur minerals. Izvestiya Vysshikh Uchebnykh Zavedeniy Geologiya i Razvedka

Epstein, L.G.; Goudsmit, J.; Paul, D.A.; Morrison, S.H.; Connor, E.M.; Oleske, J.M.; Holland, B., 1987: Expression of human immunodeficiency virus in cerebrospinal fluid of children with progressive encephalopathy. The retrovirus that causes acquired immune deficiency syndrome (AIDS) is now designated the human immunodeficiency virus (HIV). The cerebrospinal fluid (CSF) of 27 children with HIV infection was assayed for intra-blood-brain barrier (IBBB) synthe...

Urban, M.; Bommer, C.; Tennstedt, C.; Lehmann, K.; Thiel, G.; Wegner, R.D.eter; Bollmann, R.; Becker, R.; Schulzke, I.; Koerner, H., 2002: Ring chromosome 6 in three fetuses: Case reports, literature review, and implications for prenatal diagnosis. Prenatal and postnatal findings in three fetuses with a ring chromosome 6 are presented, and the literature of this rare cytogenetic disorder is reviewed. The described fetuses illustrate the broad spectrum of the clinical manifestation of ring ch...

Johnson, C.A., Iii, 1967: Sternotherus minor peltifer (Chelonia), a new host record for Heronimus chelydrae MacCallum, 1902 (Trematoda: Digenea). Heronimus chelydrae was recovered from the lungs of 4 Sternotherus minor peltifer taken in Alabama, U.S.A. This is a new host record. The name H. mollis proposed by Stunkard (1964) for H. chelydrae is not accepted by the author.

Link, J.T.; Sorensen, B.K., 2000: A method for preparing C-glycosides related to phlorizin. Johnson's Suzuki coupling protocol was employed to prepare C-glycoside analogs of phlorizin (1). In vitro biological evaluation of these C-glycosides indicated the anomeric oxygen is important to the SGLT inhibitory activity of phlorizin (1)...

Bock, H.D., 1952: Leucoplacia of tongue. Dermatologische Wochenschrift 125(3): 57-57