geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

miRNA-106a directly targeting RARB associates with the expression of Na(+)/I(-) symporter in thyroid cancer by regulating MAPK signaling pathway


, : miRNA-106a directly targeting RARB associates with the expression of Na(+)/I(-) symporter in thyroid cancer by regulating MAPK signaling pathway. Journal of Experimental & Clinical Cancer Research 35(1): 101-101

Serum miRNAs profiles between papillary thyroid carcinoma (PTC) patients with non-(131)I and (131)I-avid lung metastases are differentially expressed. These miRNAs have to be further validated and the role of these miRNAs in the molecular function level of thyroid cancer cell lines has not been investigated. Expression levels of six identified miRNAs were assessed via quantitative real-time PCR (qRT-PCR) in the serum of eligible patients. Dual-luciferase reporter assay was used to determine the potential target of miR-106a. Cell viability and apoptosis were evaluated by MTT assay and flow cytometry analysis, respectively. The change of gene expression was detected by qRT-PCR and western blotting analysis. In vitro iodine uptake assay was conducted by a γ-counter. Compared to PTC patients with (131)I-avid lung metastases, miR-106a was up-regulated in the serum of patients with non-(131)I-avid lung metastases. The results of dual-luciferase reporter assay demonstrated that miR-106a directly targeted retinoic acid receptor beta (RARB) 3'-UTR. miR-106a-RARB promoted viability of thyroid cancer cells by regulating MEKK2-ERK1/2 and MEKK2-ERK5 pathway. miR-106a-RARB inhibited apoptosis of thyroid cancer cells by regulating ASK1-p38 pathway. Moreover, miR-106a-RARB could regulate the expression of sodium iodide symporter, TSH receptor and alter the iodine uptake function of thyroid cancer cells. miRNA-106a, directly targeting RARB, associates with the viability, apoptosis, differentiation and the iodine uptake function of thyroid cancer cell lines by regulating MAPK signaling pathway in vitro. These findings in the present study may provide new strategies for the diagnosis and treatment in radioiodine-refractory differentiated thyroid carcinoma.

US$19.90

PMID: 27342319

DOI: 10.1186/s13046-016-0377-0


Other references

Ding, L.; Chen, F., 2017: Precancerous Clonal Expansion: A New Therapeutic Target?. Journal of Clinical Oncology 35(14): 1503-1505

Kalmár Nagy, Károly.; Baumann, János.; Szakály, Péter.; Gyori Molnár, Ián.; Wittmann, Ián.; Lodge, P.; Horváth Ors, Péter., 2004: Simultaneous pancreas-kidney transplantation--an alternative option for the treatment of type 1 diabetes mellitus with renal failure. Simultaneous pancreas kidney (SPK) transplantation is the only routinely used therapeutic option which can provide insulin independence, euglycemia and good renal replacement. Analysis of the five years' experience of the first Hungarian SPK...

Zufall F., 1992: Neurotoxins as tools in characterization of gamma aminobutyric acid activated chloride channels. Conn, P M (Ed ) Methods in Neurosciences, Vol 8 Neurotoxins Xvii+423p Academic Press, Inc : San Diego, California, Usa; London, England, Uk Illus 323-331

Arsenian, J., 1962: Situational factors contributing to mental illness in the elderly. Geriatrics 17: 667-674

Kadota, I.; Ueno, H.; Yamamoto, Y., 2003: Convergent synthesis of the A-F ring segment of yessotoxin and adriatoxin. A convergent synthesis of the A-F ring segment of yessotoxin and adriatoxin was achieved via the intramolecular allylation of an alpha-chloroacetoxy ether and subsequent ring-closing metathesis.

Liu, H., 2016: Early Stage Non-small Cell Lung Cancer: Surgery or Stereotactic Ablative Radiotherapy?. Zhongguo Fei Ai Za Zhi 18(6): 328-329

Wigglesworth, J.S., 1988: Lung development in the second trimester. British Medical Bulletin 44(4): 894-908

Ko, W.J.; Chou, N.K.an; Hsu, R.B.n; Chen, Y.S.arng; Wang, S.S.en; Chu, S.H.un; Lai, M.Y.ng, 2001: Hepatitis B virus infection in heart transplant recipients in a hepatitis B endemic area. Background: Hepatitis B virus (HBV) infection is hyperendemic in Taiwan. It is almost impossible for us to reject organ donors or recipients with positive serum hepatitis B surface antigen (HBsAg). We report our experience with HBV infection in he...

Turton M.B.; Matthews H.R., 1974: Catecholamines and peripheral vaso constriction after open heart surgery. Clinica Chimica Acta 50(3): 419-423

Kim, H.S.; Goncalves, N.; Henson, J.W., 1999: Cell-specific regulation of the glial-specific JC virus early promoter by large T antigen. Society for Neuroscience Abstracts 25(1-2): 1816